Description

This product is composed of 11 bands of double-stranded DNA and is suitable for the analysis of DNA bands in agarose gel electrophoresis. This product is a ready-to-use product, which already contains 1×loading buffer. It can be directly used for 2-5μl electrophoresis according to the needs of the experiment. It is easy to use and the electrophoresis image is clear. The 11 bands in this product are 100,200,300,400,500,600,700,800,900,1000,1500 bp, of which 500 ng band is 100 ng/5 μl, and the remaining bands are 50 ng/5μl.

specifications: 100T (2 × 250μl)

Storage conditions: 4 ℃ storage (long-term storage at -20℃).

Instructions:

1. Take 2-5μl of this product and add it to the well of the agarose gel for electrophoresis.

Note: The amount of sample is determined according to the thickness and width of the electrophoresis comb. 1μl was loaded per 1 mm × 1 mm (thickness × width) well; 2μl was applied to a narrow-toothed comb (typically 1 mm × 2mm); 5μl was loaded on a wide-toothed comb (typically 1mm × 5mm). If a thick-toothed comb or a comb wider than 5 mm is used, the amount of the sample can be appropriately adjusted.

2. Recommended electrophoresis conditions: gel concentration 2.0-2.5%, gel length 5-7cm, electrophoresis voltage 4-10v/cm, electrophoresis time 20-25 minutes.
3. Observe the band under UV light after EB staining.

Note: If dyeing is performed using a dye such as Goldview, since the sensitivity is lower than EB, please increase the Marker's sample loading as appropriate.

Precautions:

1. The quality of agarose has a great influence on the electrophoresis of DNA. When using electrophoresis, please use agarose with excellent quality.
2. Use freshly prepared running buffer and freshly prepared agarose gel for electrophoresis to ensure good Marker separation.
3. The Marker is suitable for the determination of DNA fragment size and accurate quantification of DNA content.