Description
This kit uses a competitive ELISA method. The microtiter plate is coated with bacitracin-conjugated antigen, and a bacitracin standard or sample is added. Compete for anti-bacteriocin antibody markers, develop color with TMB substrate, change the color from blue to yellow after adding the stop solution, and use a microplate reader to detect at 450nm. The absorbance is inversely proportional to the bacitracin content in the sample. The content of bacitracin in the sample was calculated from the standard curve.
Reactivity :This kit is used for the quantitative detection of bacitracin residues in microorganisms, feed, fish, shrimp and meat tissues (such as chicken, beef and pork), eggs, honey, milk, food, serum and urine.
Method type : competitive ELISA method
Minimum Detection Limit :0.05ppb
Detection Range :0.1 ppb to 8.1 ppb
Application : ELISA
Purpose : For the quantitative determination of target substances concentrations.
Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other
Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates
Plate : Pre-coated,Strips (12 x 8)
Restrictions : For Research Use only
Storage : 2 °C - 8 °C
Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date : 12 months
Size : 96T
Availability : 3-5 working days
Precision : Intra-assay Precision (Precision within an assay) CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%<15% Three samples of known concentration were tested in twenty assays to assess.
Linearity : To assess the linearity of the assay, samples were spiked with hig3h concentrations of rat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Recovery :
Typical Data : These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 1000 0.088 0.090 0.089 500 0.135 0.142 0.139 250 0.227 0.237 0.232 125 0.324 0.341 0.333 62.5 0.583 0.598 0.591 31.25 0.847 0.864 0.856 15.62 1.228 1.235 1.232 0 2.155 2.199 2.177
