Description
This product consists of 12 bands of double-stranded DNA bands for the analysis of DNA bands in agarose gel electrophoresis. This product is a ready-to-use product, which already contains 1×loading buffer. It can be directly used for 2-5μl electrophoresis according to the needs of the experiment. It is easy to use and the electrophoresis image is clear. The 12 strips in this product are 300,500,800,1000,1500,2000,3000,4000,5000,6000,8000,10000bp. The content of the 2000 bp band was 100 ng/5 μl, and the concentration of the remaining bands was 50 ng/5 μl.
specifications: 100T (2 × 250μl)
Storage conditions: 4 ℃ storage (long-term storage at -20℃).
Instructions:
- Take 2-5μl of this product and add it to the well of the agarose gel for electrophoresis.
Note: The amount of sample is determined according to the thickness and width of the electrophoresis comb. 1μl was loaded per 1 mm × 1 mm (thickness × width) well; 2μl was applied to a narrow-toothed comb (typically 1 mm × 2mm); 5μl was loaded on a wide-toothed comb (typically 1mm × 5mm). If a thick-toothed comb or a comb wider than 5 mm is used, the amount of the sample can be appropriately adjusted.
- Recommended electrophoresis conditions: gel concentration 2.0-2.5%, gel length 5-7cm, electrophoresis voltage 4-10v/cm, electrophoresis time 35-40 minutes.
- Observe the band under UV light after EB staining.
Note: If dyeing is performed using a dye such as Goldview, since the sensitivity is lower than EB, please increase the Marker's sample loading as appropriate.
Precautions:
- The quality of agarose has a great influence on the electrophoresis of DNA. When using electrophoresis, please use agarose with excellent quality.
- Use freshly prepared running buffer and freshly prepared agarose gel for electrophoresis to ensure good Marker separation.
