Description

Canine parvovirus disease is a potent infectious disease of dogs with high levels of contagious infection. Clinically characterized by acute hemorrhagic enteritis and myocarditis. The canine parvovirus (CPV) antigen rapid detection card uses a rapid immunochromatographic assay to detect canine parvovirus antigens. After the rectal or fecal sample is added to the well, it is moved along the chromatographic membrane along with the colloidal gold-labeled anti-CPV monoclonal antibody. If a CPV antigen is present in the sample, it binds to the antibody on the test line to display wine red. If the CPV antigen is not present in the sample, no color reaction occurs.
Assay Procedure:
1. Use a cotton swab to collect freshly excreted stool samples or sample directly from the rectum. If you are screening for parvovirus in dogs without diarrhea, use rectal sampling. It is not recommended to collect dry stool samples because the collection of dry feces often results in false positives due to excessive sample collection.
2. Immediately insert the cotton swab into the collection tube containing the sample buffer, rotate the cotton swab repeatedly on the tube wall at least 10 times, and mix the solution to dissolve the sample as much as possible in the solution. Squeeze the cotton swab on the tube wall to allow the liquid to be squeezed out as much as possible and discard the cotton swab.
3. If the stool particles are large, it can be allowed to stand for 1-2 minutes to precipitate large particles.
4. Remove the test card from the sealed bag. Place the test card on a flat surface, use a pipette to draw the upper clear liquid from the collection tube, and slowly and drop 5 drops to the well with the“S”mark.
5. At this point, you will see the wine-red liquid flowing through the observation window. If the liquid does not flow, you can gently press the area between the “sample hole” and the “observation window” to help release the liquid.
6. Leave the test at room temperature for 10-15 minutes. The result of more than 15 minutes is invalid.
Result Analysis:
1. Positive: In the observation hole, the detection line region (T) and the control line region (C) simultaneously appear a fuchsia line.
2. Negative: Within the observation well, only a reddish line appears in the control line (C).
3. Failure: In the observation hole, no color line appears in the control line area (C) and the detection line area (T); or only the detection line area (T) appears color line.
Specification: 20T
Valid Period: The kit is stored at 2～30°C, the validity period is 24 months.