Description

Purity:> 95 % as determined by SDS-PAGE.

Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method.

Biological activity:Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1200 pmol/min/μg.

Protein construction:A DNA sequence encoding the human FAP isoform 1 (Q12884-1) extracellular domain (Leu 26-Asp 760) was fused with the polyhistidie-tag at the N-terminus.

Host:HEK293 Cells

Species:Human

Prediction N:His

Molecular weight:The recombinant human FAP consists of 751 amino acids and predicts a molecular mass of 87.2 kDa. As a result of glycosylation, rh FAP migrates as approximately 90 kDa band in SDS-PAGE under reducing conditions.

Buffer:

Lyophilized from sterile 25mM Tris, 250mM NaCl, pH 8.2.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please refer to the specific buffer information in the hard copy of CoA.

Stability & storage conditions:

Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Specification:1oug