Description

The kit uses a one-step method to carry out RT and PCR in the same reaction system, no need to add reagents during the reaction process, no need to open the tube cover, avoiding cross-contamination between samples and improving the sensitivity of detection.
The 25×RT-PCR Enzyme Mix in this kit is a pre-mixed Mix form of TIANGEN's new reverse transcriptase (King RTase), antibody-modified hot-start Taq DNA polymerase and RNase Inhibitor. The King RTase is molecularly modified. The novel reverse transcriptase specifically increases the hydrophobic motif, has stronger RNA affinity and thermal stability, and improves the reverse transcription efficiency of the enzyme and the extension ability of the RNA template with complex secondary structure; The high-performance antibody-modified Taq DNA polymerase, wherein the high-affinity antibody can ensure complete blocking of Taq enzyme activity under normal temperature conditions, so that the post-reverse PCR reaction has higher amplification efficiency and specificity. In addition, the 2×FastKing One Step RT-PCR MasterMix in this product is a new reaction system optimized for the above two key enzymes, including essential ionic components, dNTPs and PCR stabilizers and enhancers to ensure King RTase And Taq polymerase maximizes efficacy throughout the one-step reaction.

Characteristics：

Purity: reverse transcription and PCR reactions are completed in one step to avoid cross-contamination

Efficient: unique King reverse transcriptase with over 95% inversion efficiency

Sensitive: Templates as low as 1 ng can also be accurately identified, especially for low abundance templates

Specific: Antibody-modified Taq enzyme, again increasing amplification efficiency and specificity.

Specification:50t