Description
The kit takes out from the refrigeration should be balanced 15-30 minutes in the room temperature, if the coated ELISA plates have not been used up after opening, the plate should be stored in sealed bag.
Washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilution. Washing does not affect the result.
Pipette sample with pipettors each step, and proofread its accuracy frequently, avoids the experimental error. Pipette sample within 5 min, if the number of sample is much, recommend using multichannel pipettor.
If the testing material content is excessively high (The sample OD is higher than the first standard well)?please dilute sample (n-fold).
Adhesive Strip only limits the disposable use to avoid cross-contamination.
The substrate should be preserved evade the light.
Please refer to use instruction strictly. The test result determination must take the microtiter plate reader as a standard.
All samples, washing buffer and each kind of reject should refer to infective material process.
Do not mix reagents with those from other lots.
Reactivity : Goat
Method type : Sandwich ELISA
Minimum Detection Limit :
Detection Range :
Application : ELISA
Purpose : For the quantitative determination of target substances concentrations.
Research Area : Tags & Cell Markers->Cell Type Markers->Tumor Associated, Signal Transduction->Cytoskeleton / ECM->Cell Adhesion->Cell Adhesion Molecules->Vascular, Signal Transduction->Cytoskeleton / ECM->Cell Adhesion->Cell Adhesion Molecules->Endothelial, Neuroscience->Neurology process->Neural Signal Transduction, Stem Cells->Mesenchymal Stem Cells->Surface Molecules, Cancer->Invasion/microenvironment->ECM->Cell adhesion->Other, Cardiovascular->Atherosclerosis->Vascular Inflammation->Leukocyte recruitment->Cell adhesion molecules, Kits/ Lysates/ Other->Kits->ELISA Kits->ELISA Kits->Adhesion molecules ELISA kits, Kits/ Lysates/ Other->Kits->ELISA Kits->ELISA Kits->Atherosclerotic proteins ELISA kits, Cardiovascular->Angiogenesis->Endothelial Cell Markers
Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates
Plate : Pre-coated,Strips (12 x 8)
Restrictions : For Research Use only
Storage : 2 °C - 8 °C
Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date : 12 months
Size : 96T
Uniprot No. :A0A1C9FRA0
Abbreviation :
MDSLSVNQVLYPEVHLDSPIVTNKLVAILEYSGIDHNYVLEDQTLVKNIRYRLGCGFSNQ
MIINNRGVGETVNSKLKSYPHNRHIIYPDCNKELFCIKDSCISKKLSELFKKGNSLYSKI
SHQVLDCLKRVNGKLGLGTDLTHGLKEGILDLGLHMHSSQWFETFLFWFTIKTEMRSMIK
EQSHICHKRRYNPTFVSGDAFEVLVSRDLVVIIDKNTQYVFYLTFELVLMYCDVIEGRLM
TETAMAIDQRYSELLSRVRYLWDLIDGFFPTLGNTTYQIVALLEPLSLAYLQLQDVTLEL
RGAFLDHCFKELYEILEHCGIDTAGTYNSITEGLDHVFITHDIHLTGEIFSFFRSFGHPR
LEAVTAAENVRKHMNQPKVISYETMMKGHAVFCGIIINGFRDRHGGSWPPVALPEHASAA
IRNAQASGEGLTHDLCIDNWKSFVGFKFGCFMPLSLDSDLTMYLKDKALAALKNEWDSVY
PKEYLRYNPPRGTESRRLVEVFLNDSSFDPYNMIMYVVNGSYLKDPEFNLSYSLKEKEIK
ETGRLFAKMTYKMRACQVIAENLISNGVGKYFRDNGMAKDEHDLTKALHTLAVSGVPKNN
KDNHRGGPPRRTTNRGVRSSQGTKTQDRDKVQGGPMYNYLRCQPISPDQGESYETVSAFI
TADLKKYCLNWRYETISIFAQRLNEIYGLPSFFQWLHRILEKSVLYVSDPHCPPDLDNHI
PLDSVPNAQIFIKYPMGGIEGYCQKLWTISTIPYLYLAAYESGVRIASLVQGDNQTIAVT
KRVPSSWPYSLKKREASKAAQNYFVVLRQRLHDVGHHLKANETIVSSHFFVYSKGIYYDG
LLVSQSLKSIARCVFWSETIVDETRAACSNIATTVAKSIERGYDRYLAYSLNILKIFQQI
LISLNFTINTTMTQDVVAPIIENGDLLIRMALLPAPIGGLNYLNMSRLFVRNIGDPVTSS
IADLKRMIDAGLMPEETLHQVMTQTPGESSYLDWASDPYSANLPCVQSITRLLKNITARY
ILISSPNPMLKGLFHEGSRDEDEELASFLMDRHIIVPRAAHEILDHSITGAREAIAGMLD
TTKGLIRTSMKRGGLTPRVLARLSNYDYEQFRSGITLLTKKGQCYLIDKDSCSVQLAIAL
RGHMWAKLARGRPIYGLEVPDVLESMNGYLIKRHESCAICETGSSHYGWFFVPAGCQLDD
VSRETSALRVPYVGSTTEERTDMKLAFVRSPSRSLKSAVRIATVYSWAYGDDEKSWSEAW
MLARQRANITLDELRMITPVSTSTNLAHRLRDRSTQVKYSGTSLVRVARYTTISNDNLSF
VISEKKVDTNFIYQQGMLLGLGILENLFRLEATTGVSNTVLHLHVETECCVVPMVDHPRI
PSLRNIKVTDELCTNPLIYDRSPIIEHDATRLYSQSHRRHLVEFVTWSTSQLYHILAKST
AMSMIELITRFEKDHMNEIAALIGDDDINSFITEFLLVEPRLFIVYLGQCAAINWAFDIH
YHRPSGKYQMGELLYSLLSRMSKGVYKIFTNALSHPKVYKKFWRSGIIEPIHGPSLDTQN
LHVTVCDMIYGSYVTYLDLLLNDELDDYPYLLCESDEDVVTDRFDNIQAKHLCVLADVYC
SSKRCPSIIGMSPIEKCTILTHYIKGESVQSPSGTSWNTDPLVVDHYSCSLTYLRRGSIK
QIRLRVDPGFVFEALTDVDFKQPRKAKLDISVVGLTDFSPPYDNVGDFLGTINTLRHDLP
VTGTGVSNYEVHAYRRIGLNSSACYKAVEISTLIXPSLEVGEHGLFLGEGSGSMLAAYKE
VLKLANCYYNSGVTAEGRAGQREISPYPSEMSLVENQMGIERSVKVLFNGKPEVTWVGTT
DCYKYIISNIQTSSLGFIHSDIETLPTKDAVEKLEEFASILSLSLILGKIGSITVVKIMP
ISGDFTQGFIAYAIQYFRESLLAYPRYSNFISTECYLIMIGLKANRLINPEAIKQSIIRA
GVRTAPGLVSHVLSEKQKGCIQSFLGDPYIQGDFNKHLKSLTSIEKILVNCGLSINGIKI
CRDLIHHDIASGPDGLMSSTIILYRELAHFKDNIRSQHGMFHPYPVLANSRQRELILRIA
KKFWGYVLLYSDDPALIRQTIKNLKRNHLTFDLHSNPFIKGLSKAEKLLVRTSSLRREWL
FTLETKEVKEWFKLVGYSALIRG
Availability : 3-5 working days
Target Details :RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Precision : Intra-assay Precision (Precision within an assay) CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%<15% Three samples of known concentration were tested in twenty assays to assess.
Linearity : To assess the linearity of the assay, samples were spiked with hig3h concentrations of rat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Recovery :
Typical Data : These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 1000 0.088 0.090 0.089 500 0.135 0.142 0.139 250 0.227 0.237 0.232 125 0.324 0.341 0.333 62.5 0.583 0.598 0.591 31.25 0.847 0.864 0.856 15.62 1.228 1.235 1.232 0 2.155 2.199 2.177
