Description

Source: Prokaryotic expression.

Specification：50ug

Host: E. coli
Residues: Ile523~Asp760
Tags: N-terminal His-Tag
Tissue Specificity: Adipose, Fetal skin, Glioma, HSC. Subcellular Location: Cell surface. Cell membrane; Single- pass type II membrane protein. Membrane, Single-pass type II
membrane protein. Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose. Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 30.5kDa
Accurate Molecular Mass: 26&40kDa as determined by SDS-PAGE reducing
conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene

2. Relative charge: The composition of amino acids may affects the charge of theprotein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.