Description

Taq DNA Polymerase was isolated and purified from Escherichia coli cloned with Thermu aquaticus DNA Polymerase gene and has a molecular weight of 94 KD. It has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity, and has no 3'-5' exonuclease activity. In the PCR reaction, Real Taq DNA Polymerase has an extension rate of 1-2 kb/min, and the product 3' end carries A, which can be directly cloned using the TA vector. 1 unit (U) Real Taq DNA Polymerase activity is defined as the incorporation of 10 nmol deoxynucleotides into acid insoluble materials using activated salmon salmon sperm DNA as a template/primer at 74 ° C for 30 minutes. The amount of enzyme.

Product number and specifications :

Storage conditions: -20℃.

Concentration: 5U/ul

Enzyme storage buffer: 20 mM Tris-HCl (pH 8.0);0.1 mMEDTA;1 mMDTT;100 mMKCl ; Stabilizers; 50% glycerol.

10×Taq Buffer: 200 mM Tris-HCl (pH 8.4);200 mMKCl;15 mMMgCl2; Other ingredients.

Scope of application: generally used for PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, blunt end plus A, etc., the product can be directly used for T/A vector cloning.

Reaction examples: The following examples are conventional PCR reaction systems and are for reference only. The actual reaction conditions vary depending on the structure of the template, the primer, etc., and the optimal reaction conditions are set according to the specific conditions such as the size of the template, the size of the target fragment, the base sequence, and the length of the primer.

Establishment of the reaction system: 50 ml of the reaction system is as follows (the reaction system can be scaled up or down according to the ratio):